No Nucleotide Sequence Variation at Non-Coding Mitochondrial DNA of Cassava (Manihot esculenta Crantz)
Abstract
Cassava (Manihot esculenta Crantz) is an important economic crop of Thailand. The purpose of this study is to investigate the non-coding mitochondrial DNA region of cassava that has not yet been reported for use as a molecular marker for cultivar identification. In this study, 18 cassava cultivars were used to obtain the 368-bp segment using trn-Phe and trn-F primers in the Polymerase Chain Reaction. The non-coding mitochondrial DNA region of all samples were 268 base pairs, with no nucleotide difference. Comparison between sequences of other members within Euphorbiaceous (same family as cassava), Para rubber (Hevea brasiliensis) and castor bean (Ricinus communis), revealed 10 and 33 base pairs being different, respectively. Moreover, when compared to mung bean (Vigna radiata (L.)), out of Euphorbiaceous family, found 32 base pairs different. Therefore, the non-coding mitochondrial DNA region is not suitable for cassava identification at the cultivar level.
Keywords : DNA, cassava, mitochondrial DNA, PCR
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