In vitro Shoot Induction and Shoot Multiplication of Asparagus (Asparagus officinalis)
Abstract
From shoot induction and shoot multiplication of Asparagus (Asparagus officinalis) in vitro was launched by cultivating 1.5±0.2 cm lateral bud on Murashige and Skoog (MS, 1962) media with the addition of plant growth regulators in cytokinin group i.e. BAP at 0, 0.5, 1.0, 1.5 and 2.0 mg/L. After culturing for 4 weeks, it was found that MS media with BAP in all concentration could induce shoot as well. In addition, some lateral bud explants could be developed into callus. Obviously, MS media with 0.5 mg/L BAP had the highest shoot length and statistically different from other experiments. After that, shoot tip explants were obtained from aseptic seedlings culturing on MS media supplemented with BAP or kinetin alone or together with NAA at 0.1, 0.5, 1.0 mg/L. The result showed that MS medium supplemented with 0.5 mg/L BAP or 1.0 mg/L kinetin alone or addition of 0.5 mg/L BAP with 0.1 mg/L NAA could induce number of shoot well. For root induction, shoot tip explants were cultured on MS media supplemented with plant growth regulators in auxin group including NAA, IAA and IBA at different concentration for 8 weeks. The result revealed that MS media supplemented with 1.0 mg/L IBA was more effective in root induction of Asparagus while other experimental, no root formation.
Keywords : shoot and root induction ; plant growth regulator ; Asparagus officinalis.
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