The Influence of Plant Growth Regulators and Chitosan  on Drosera adelae F.Muell. In Vitro Condition

Sirasatiyakorn ฺBanharn; Chorthip Ngoentem

The Influence of Plant Growth Regulators and Chitosan on Drosera adelae F.Muell. In Vitro Condition

Sirasatiyakorn ฺBanharn, Chorthip Ngoentem

Abstract

The objective of this research was to investigate the influence of plant growth regulators and chitosan on micro-propagation of New Zealand sundew (Drosera adelae F. Muell.). The 0.5 × 0.5 cm² leaf explants of New Zealand sundew seedlings from 3-month-old were cultured on ½ MS (Murashige and Skoog, 1962) agar media with kinetin at concentrations of 0 0.1 and 0.2. mg/l together with 2, 4- D at 1, 3 and 5 mg/l concentrations for 5 weeks. From the results, it was found that leaf explants cultured on ½ MS agar media containing 0.2 mg/l kinetin with 1.0 mg/l 2, 4-D could induce compact callus induction 100% with average diameter of 15.60 mm and the maximum fresh weight of 0.36 g calluses. Then, the callus was cultured on ½ MS agar media containing with kinetin at the concentration of 0, 0.2, 0.4, 0.6, 0.8 and 1.0 mg/l for 7 weeks. It was found that ½ MS media containing 0.4 mg/l kinetin could induce shoot well with an average shoot number 20.00 shoot/explant, average shoot height 1.30 centimeters, average number of leaves 4.80 per shoot and the average leaf length 1.90 centimeters. For root induction, the shoot explant was cultured on ½ MS media containing NAA at the concentration of 0, 0.25, 0.50, 0.75 and 1.0 mg/l for 7 weeks. From the result, it was found that ½ MS media containing 0.75 mg/l NAA promoted the induction of root well with the average root number 15.80 root/shoot. While the cultivation of callus on ½ MS media containing chitosan at 0, 0.2, 0.4, 0.6, 0.8 and 1.0 % v/v for 5 weeks, it was found that ½ MS agar media containing 0.8 % v/v chitosan could induce callus 100% and gave the average highest root number of 13.00 root/shoot. Keywrods : chitosan ; in vitro ; plant growth regulator ; New Zealand Sundew ; Drosera adelae F.Muell

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