Production of Monoclonal Antibody Specific to Immunoglobulin of Asian Sea Bass Lates calcarifer
Abstract
Production of monoclonal antibodies (MAbs) specific to Asian sea bass immunoglobulin (Ig) was prepared by peritoneal injection the Asian sea bass with Bovine serum albumin (BSA) first. Then the fish anti-BSA antiserum was used to precipitate with BSA by immunoprecipitation method. The Ig/BSA complex was then immunized into mice. The mouse that showed the strongest immune response against Asian sea bass Ig was used for monoclonal antibody production. After fusion of myeloma with B cells of mice, 3 hybridoma clones (4C7, 6H7 and 15C8) producing monoclonal antibody specific to heavy chain (~80 kDa) of Asian sea bass Ig were selected by dot blot and Western blot, however the MAb (4C7) showed slightly cross reaction with tilapia serum.
Keywords: monoclonal antibody, Lates calcarifer, immunoglobulin, double immunodiffusion, Western blot
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