Ultrastructure of Oocyte Development in Anemone Clownfish, Amphiprion ocellaris (Cuvier, 1830)
Abstract
Histological studied the stages of oocytes development in Amphiprion ocellaris was classified into 5 stages based on nuclear characteristics, cytoplasmic content and zona radiata layers; chromatin nucleolar (OC1) stage, perinucleolar stages (OC2), cortical alveoli and oil droplets (OC3) stage, vitellogenesis stage (OC4) and maturation stage (OC5). According to ultrastructural studied, oogonium was characterized by a large nucleus with a prominent nucleolus. The chromatin-nucleolar stage (OC1), there was cytoplasmic differentiation, with the formation of Balbiani’s body (mitochondrial cloud). The perinucleolar stage (OC2), oocyte’s nucleus contained multiple nucleoli that were located around the inner membrane of germinal vesicle envelope. Electron dense material (nuage) was usually observed close to outer membrane of germinal vesicle envelope. Cortical alveoli and oil droplets stage (OC3), oocytes were distinguished by appearance of many cortical alveoli-like vesicles and oil droplets in cytoplasm. In this stage, zona radiata was observed as a thin layer around the oocyte . In vitellogenic stage (OC4), oocytes were larger, yolk granules containing a dense homogenate material were abundant in the cytoplasm. During maturation stage (OC5), the yolk granules fused to become yolk platelets in cytoplasm and the thickness of zona radiata were greatest, and divided into zona radiata interna (ZRi) and zona radiata externa (ZRe).
Key words : Amphiprion ocellaris, oocyte, transmission electron microscopy
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