Differences in Genetic Characteristics and Seed Proteomes of  Oryza sativa  ‘Khao Dawk Mali 105’ and Its Mutant ‘Mali Daeng’

Sarunyaporn Maksup; Sarintip Pongpakpian; Sittiruk Roytrakul; Kanyaratt Supaibulwatana

Differences in Genetic Characteristics and Seed Proteomes of Oryza sativa ‘Khao Dawk Mali 105’ and Its Mutant ‘Mali Daeng’

Sarunyaporn Maksup, Sarintip Pongpakpian, Sittiruk Roytrakul, Kanyaratt Supaibulwatana

Abstract

Rice (Oryza sativa L.) is one of the economic grains that are used as important sources of carbohydrates in the world. A fragrant cultivar ‘Khao Dawk Mali 105’ (KDML105) is the most popular rice among Thai and international traits. It is therefore, commonly implement as the main rice genetic resource for breeding program in order to improve novel properties of trait with higher productivity and better nutritional values. ‘Mali Daeng’ (MD) rice has been previously identified as KDML105 mutant, which was characterized by its red pericarp at seed coat. However, there was no previous report showing clear identification of the genetic differences between both cultivars. This present research is the first report that reveals the structural genetic differences between KDML105 and MD rice by using simple sequence repeats (SSRs) marker. On the other hand, the differences of proteomic patterns detected in grains using GeLC-MS/MS shotgun proteomics were clarified. The results showed that KDML105 and MD had different genetic characteristics. The differences could be found on the chromosome 4, 7 and 10 by using the SSRs molecular markers from 4 pairs of primers, namely RM241, RM11, RM481 and RM304. Moreover, different protein expression profiles were observed in rice seeds. Among the 342 identified proteins, the expression of proteins (3-ketoacyl carrier synthase III, UDP-glucose flavonoid 3-O-glucosyltransferase and glycosyltransferase family 61 protein) that related to biosynthesis of fatty acids, phenolic compounds (flavonoids) and antioxidant activity were increase in MD. The results suggested that both varieties had different genetic backgrounds and can be distinguished by using SSRs molecular markers and MD may have higher nutritional value than KDML105 rice and suitable for promotion of planting for rice export.

Keywords: Khao Dawk Mali 105, Mali Daeng, molecular markers, SSRs, proteomics

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