Development of Multi-Assay Paper-Based Devices for Analysis of Antioxidant Activity
Abstract
This work developed a paper-based device for simultaneous determination of multiple antioxidant activity assays including the cupric reducing antioxidant capacity (CUPRAC) assay, the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) assay, and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonate) radical cation (ABTS) assay. The device composed of a central sample zone connected to four detection zones to accommodate three antioxidant assays and a sample blank measurement. Antioxidant activity analysis was achieved by dropping the samples onto the sample zone to flow to the detection zones containing the stored reagents for each antioxidant assay making the change in color that was measured using image J software. The analysis of gallic acid antioxidant standard with CUPRAC, ABTS, and DPPH assay gave the calibration curve in the linear ranges of 1-6 mM, 20-150 µM, and 3-13 mM, respectively, the relative standard deviation from the repetitive analysis of gallic acid at the concentrations in the linear range are 0.70-1.61%, 0.91-4.04% and 1.39-4.91% (n=5), respectively, and a limit of detection of 1 mM 1.10 µM and 1.30 mM, respectively. These preliminary results indicated that the developed paper-based device provided for the analysis of multiple antioxidant assays at the same time with low analysis time and cost, low reagent consumption and is promising to use for antioxidant activity in real samples.
Keywords: multi-assay analysis, antioxidant activity, antioxidant, paper-based devices
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