Oogenesis and Localization of Progesterone Receptors in Ovary of Oratosquilla nepa
Abstract
The objective of this study was to describe histological characteristics of the female germ cells and to characterize progesterone receptors expression in the ovary of Oratosquilla nepa. The histology of the ovary demonstrated subsequent stages of development of the oocytes. According to their cellular characteristics, oogonia and 5 types of oocytes were classified : early chromatin nucleolus (Oc1), late chromatin nucleolus (Oc2), previtellogenic oocyte (Oc3), vitellogenic oocyte (Oc4) and mature oocyte (Oc5). Oogonium has condensed basophilic heterochromatin which is usually located near the nuclear membrane. Oc1 has a round nucleus containing dispersed heterochromatin. Oc2 has a round nucleus with 1-2 nucleoli, basophilic cytoplasm. Oc3 are larger, cytoplasm fill with vacuolated globules. Oc4 is characterized by the appearance of yolk granules in cytoplasm. Oc5 is the largest of the germ cells. The cytoplasm is filled with yolk plates stained orange color with hematoxylin-eosin. Progesterone receptor y immunoreactivity was detected in cytoplasm of Oc2 and follicular cells of Oc3, Oc4 and Oc5. In contrast, there was no progesterone receptor immunoreactivity was observed in oogonia and Oc1.
Keywords: Oratosquilla nepa, oocyte development, progesterone receptor
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