Screening of Carotenogenic Yeasts from Natural Soil at the University of Phayao

Supaporn Passorn, Nittaya Sookwanna, Panitnart Auputinan


This study aimed to isolate carotenogenic yeasts from natural soil at the University of Phayao. Three isolates of yeasts displayed orange or pink colonies were obtained after screening on Potato dextrose agar (PDA) plates, which were named as UP11 UP12 and UPF2. Internal transcribed spacer (ITS)-based identification and similarity search upon GenBank database highly specified UP12 and UPF2 as Rhodotorula mucilaginosa. In contrast,              the taxonomic position of UP11 was obscured since there was no reasonable matching sequence in the database. This observation suggested that alternative molecular chronometer should be taken into consideration.                           The optimum temperature for carotenoid production for both UP11 and UP12 was 30 oC, which were announced      as 28.59±1.69 and 14.59±0.55 µg.g-1 cell dry weight, respectively.    

Keywords :  carotenoid,  carotenogenic yeast, University of Phayao

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