Effects of Cytokinnin and Sucrose on Tissue Culture of Rosa chinensis Jacq.var. minima Voss.

Ampika Tibkwang, Sirasatiyakorn Junkasiraporn, Kittisak Chotikadachanarong

Abstract


The effects of cytokinin & sucrose on tissue culture of fairy rose (Rosa chinensis Jacq.var. minima Voss.) was conducted in sterile conditions. Nodal segments were cultured on Murashige & Skoog (MS) medium supplemented with different concentrations (0, 0.5, 1.0, 1.5, 2.0  mg/L) of 6-Benzyladenine (BA), Thidiazuron (TDZ) & Kinetin (KN) at  25±2 ºC & 16 hours of light per day with light Intensity at 1,200 lux for 4 weeks. From the result, it was found that all experiment could induce shoot 100%. Specifically, MS supplemented with TDZ at a concentration of 1.5 mg/L had the highest average number of shoots was 4.50±1.93 shoots/explant & significantly different from the other treatments. While MS medium supplemented with 1.5 mg/L TDZ could induce the highest average shoot length of 5.89±2.34 mm & statistically significant differences with other groups.  When nodal segment were cultured on MS medium supplemented  with 1.5 mg/L TDZ & sucrose concentrations at 30, 40, 50 & 60 g/L for 6 weeks. It was shown that, nodal segments were cultured on MS supplemented with TDZ at 1.5 mg/L & sucrose concentration at 40 g/L had the highest average shoot number of 2.73-2.77 shoot/explant. However, it can be seen that MS medium supplemented with sucrose at all concentrations could induce the average shoot length without statistically significant difference & no flowering occurred.

 

Keywords :  Rosa chinensis Jacq.var. minima Voss., cytokinin, sucrose


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