Diversity of Thraustochytrids Isolated from Mangrove Leaves at Thung Prong Tong, Rayong Province

Tiwaporn Thongprasom, Sudarat Suanjit, Somtawin Jaritkhuan

Abstract


Diversity, frequency of occurrence and fatty acid content of thraustochytrids isolated from fallen leaves of 12 mangrove trees at Thung Prong Tong, Rayong Province were studied in the dry and rainy season. Three genera (7 species) were identified as genus Aurantiochytrium (5 species, Aurantiochytrium sp.1, Aurantiochytrium sp.2, Aurantiochytrium sp.3, Aurantiochytrium sp.4 and Aurantiochytrium sp.5), genus Parietichytrium (1 species, Parietichytrium sp.), genus Schizochytrium (1 species, Schizochytrium sp.2). Two species of unidentified thraustochytrids were Thraustochytriidae 6 and Thraustochytriidae 7. The average frequency of occurrence of thraustochytrids was 2.5-52.5%, which showed higher in the rainy season than that of  dry season. The average biomass of Thraustochytrids were in a range of 15.63±3.03-22.87±0.80 g/L, which the highest found in Aurantiochytrium sp.1. For fatty acid content, Aurantiochytrium sp.1 had the highest content of Arachidonic acid (20:4 n-6, ARA) and Eicosapentaenoic acid (20:5 n-3, EPA) of 0.43±0.20 mg/g dry wt. (0.1±0.04 % of total fatty acid) and 1.67±1.17 mg/g dry wt. (0.35±0.20% of total fatty acid), respectively.  While the highest amount of Docosapentaenoic acid (22:5 n-6, DPA) and Docosahexaenoic acid (22:6 n-3, DHA) were found in Aurantiochytrium sp.3 at 39.40±6.17 mg/g dry wt. (8.06±0.53% of total fatty acid) and 137.23±25.61 mg/g dry wt. (31.68±2.72% of total fatty acid), respectively. Aurantiochytrium sp.1 is the appropriate stain for further industrial uses.


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