Study of genetic diversity and marker-trait association for sweetness by Target Region Amplification Polymorphism Polymerase Chain Reaction (TRAP-PCR) in Cucumis melo L.

Sangduen Inchonbot, Chotipa Sakulsingharoj, Varaporn Sangtong, Prawit Puddhanon, Saengtong Pongjaroenkit

Abstract


Cucumis melo (C. melo L.) groups is an important plant with increasing economic value. The improvement of the varieties with great tastes like melon and resistance to insect and unfavorable weather conditions similar to Thai-melon has been needed. This research aimed to study genetic diversity and relationship between molecular markers and sweetness trait of C. melo L. by using Target Region Amplified Polymorphism Polymerase Chain Reaction (TRAP-PCR) technique. Specific primers which were designed based on the sequence of nine genes involving in metabolic pathway of sugar combining with twelve arbitrary primers were used in TRAP-PCR. It was found that 59 pairs of primers were able to amplify DNA generating total 379 amplicons. The polymorphic data was used to create dendrogram based on Jaccard’s similarity index through UPGMA. The results revealed the similarity index ranged from 0.69 to 0.95. Studied plants could be divided into 3 groups with a similarity coefficient of 0.8, corresponding to the sweetness (% brix). The 220 of polymorphic bands were detected with a percentage of 58.05. Analysis of the relationship between molecular markers and sweetness trait showed thatCM01 (X174) marker was found to correlate with the sweetness of 87.07 percent, resulting from the use of primers, Neutral invertase 1 with Sa17_800. The results of genetic diversity and developed DNA markers in this study will assist breeding processes and selection of sweetness trait in C. melo L. to be more efficient, more accurate and faster.          

 

Keywords:  Cucumis melo L., TRAP-PCR, Genetic diversity, Molecular marker, sweetness

 


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