Effects of Yeast (Saccharomyces cerevisiae) Supplements on Growth Performance and Blood Chemical Profiles of Nile Tilapia (Oreochromis niloticus) at Different Stocking Density

Nantaporn Sutthi, Supranee Wigraiboon, Pantaporn Supakankul

Abstract


Yeast (Saccharomyces cerevisiae) is a popular probiotic feed additive to promote the health of aquatic animals.  Effects of yeast supplements at 0.5% on growth performance and blood chemical profile of Nile tilapia (Oreochromis niloticus) were studied at different densities 12, 18 and 24 fish/tank (218, 327 and 436 fish/m3) over 90 days. Results determined that yeast supplement had a significant effect on survival rate (P<0.05). Fish fed with 0.5% yeast and reared at a density of 24 fish/tank showed higher survival rate (85.53±2.31%) than the control group (no added yeast) (76.00±4.00%). For blood chemical profiles, levels of cortisol, blood glucose, aspartate aminotransferase (AST), alanine aminotransferase (ALT) and malondialdehyde (MDA) of fish fed with yeast supplement and reared at a density of 24 fish/tank were significantly lower than the control group (P<0.05) at 16.39±2.27 µg/dL, 84.07±2.52 mg/dL, 65.67±2.52 unit/L, 28.33±3.11 unit/L and 78.45±7.46 µM/L, respectively. Thus, 0.5% yeast supplement added to the diet improved survival rate and blood chemical profiles of Nile tilapia despite crowding stress, suitable benefit at moderate densities 24 fish/tank.              

Keywords : Saccharomyces cerevisiae, Nile tilapia, growth performances, blood chemical profiles


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References


Abdel-Tawwab, M., Abdel-Rahman, A.M., & Ismael, N.E.M. (2008). Evaluation of commercial live bakers’ yeast, Saccharomyces cerevisiae as a growth and immunity promoter for Fry Nile tilapia, Oreochromis niloticus(L.) challenged in situ with Aeromonas hydrophila. Aquaculture, 280, 185–189.

Abdel-Tawwab, M., Mousa, M.A.A., & Mohammed, M.A. (2010). Use of live baker’s yeast, Saccharomyces cerevisiae, in practical diet to enhance the growth performance of Galilee tilapia, Sarotherodon galilaeus (L.), and its resistance to environmental copper toxicity. Journal of the World Aquaculture Society, 41, 214–223.

Barcellos, L.J.G., Nicolaiewsky, S., de Souza S.M.G., & Lulhier, F. (1999). The effects of stocking density and social interaction on acute stress response in Nile tilapia Oreochromis niloticus (L.) fingerlings. Aquaculture Research, 30, 887–892.

Barreto, R.E., & Volpato, G.L. (2006). Stress responses of the fish Nile tilapia subjected to electroshock and social stressors. Brazilian journal of medical and biological research, 39(12), 1605-1612.

Barton, B.A. & Iwama, G.K. (1991). Physiological changes in fish from stress in aquaculture with emphasis on the response and effect of corticosteroids. Annual Reviews of Fish Diseases, 10, 3-26.

Benedeczky, I., Biro, P., & Schaff, Z. (1984). The effect of 2,4-D-containing herbicide (Dikonirt) on the ultrastructure of carp (Cyprinus carpio) liver cells. Acta Biologica Hungarica, 30, 107-125.

Ble-Castillo, J.L., Carmona-Díaz, E., Méndez, J.D., Larios-Medina, F.J., Medina-Santillán, R., Cleva-Villanueva, G., & Díaz-Zagoya, J.C. (2005). Effect of a-tocopherol on the metabolic control and oxidative stress in female type 2 diabetes. Biomedicine & pharmacotherapy, 59, 290-295.

Chansue, N., Endo, M., Kono, T., & Sakai, M. (2000). The stimulation of cytokine-like protein in tilapia (Oreochromis niloticus) orally treated with β-1,3 glucan. Asian Fisheries Science, 13, 271–278.

Chiu, C.H., Cheng, C.H., Gua, W.R., Guu, Y.K., & Cheng, W. (2010). Dietary administration of the probiotic, Saccharomyces cerevisiae P13, enhanced the growth, innate immune responses, and disease resistance of the grouper, Epinephelus coioides. Fish & Shellfish Immunology, 29(6), 1053–1059.

Department of Fisheries. (2017). Fisheries Statistics of Thailand 2015. Retrieved February 19, 2018, from http://www.fisheries.go.th/strategy-stat/themeWeb/books/2558/1/yearbook2558_Rev060960.pdf

Dimitroglou, A., Merrifield, D.L., Moate, R., Davies, S.J., Spring, P., Sweetman, J., & Bradley, G. (2009). Dietary mannan oligosaccharide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout, Oncorhynchus mykiss (Walbaum). Journal of Animal Science, 87,

–3234.

EL-Boshy, M.E., EL-Asheram, A.M.M., & EL-Ghany, N.A.A. (2008). Effect of dietary beta-1, 3 glucan on immunomodulation on diseased Oreochromis niloticus experimentally infected with aflatoxin B1.

In Proceeding 8th International Symposium on Tilapia in Aquaculture. (pp. 1109-1127). Egypt: Cairo.

EL-Khaldi, A.T.F. (2010). Effect of different stress factors on some physiological parameters of Nile tilapia (Oreochromis niloticus). Saudi Journal of Biological Sciences, 17, 241-246.

Fairchild, E.A. & Howell, W.H. (2001). Optimal stocking density for juvenile winter flounder Pseudopleuronectes americanus. Journal of the World Aquaculture Society, 32(3), 300-308.

Fernandez, F., Hinton, M. & Van Gils, B. (2002). Dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to Salmonella enteritidis colonization. Avian Pathology, 31(1), 49–58.

Giannenas, I., Karamaligas, I., Margaroni, M., Pappas, I., Mayer, E., Encarnação, P., & Karagouni, E. (2015). Effect of dietary incorporation of a multi-strain probiotic on growth performance and health status in rainbow trout (Oncorhynchus mykiss). Fish Physiology and Biochemistry, 41,119-128.

Hardie, L.J., Chappell, L.H., & Secombes, C.J. (1994). Human tumor necrosis factor α influences rainbow trout Oncorhynchus mykiss leukocyte responses. Veterinary Immunology and Immunopathology, 40(1),

–84.

Hassaan, M.S., Soltan, M.A. & Ghonemy, M.M.R. (2014). Effect of synbiotics between Bacillus licheniformis and yeast extract on growth, hematological and biochemical indices of the Nile tilapia (Oreochromis niloticus). Egyptian Journal of Aquatic Research, 40, 199-208.

Imjai, P. Chaiyara, A. & Thowanna, C. (2016). Efficiency of Streptococcus agalactiae vaccine in Nile tilapia (Oreochromis niloticus) with different stocking density. Prawarun Agricultural Journal, 13(1), 79-87.

Irianto, A. & Austin, B. (2002). Probiotics in aquaculture. Journal of Fish Diseases, 25(11), 633-642.

Iwashita, M.K., Nakandakare, I.B., Terhune, J.S., Wood, T., & Ranzani-Paiva, M.J. (2015). Dietary supplementation with Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus oryzae enhance immunity and disease resistance against Aeromonas hydrophila and Streptococcus iniae infection in juvenile tilapia Oreochromis niloticus. Fish & Shellfish Immunology, 43(1), 60-66.

Kannika, K., Panprommin, D., Whangchai, N., & Chitmanat, C. (2012). Effects of brewer’s yeast (Saccharomyces cerevisiae) supplementary diet on growth performance and immune response in Nile tilapia.

In Proceedings of 49th Kasetsart University Annual Conference: Fisheries (pp. 408-415.) Bangkok.

(in Thai)

Kardsakun, P., Chaibu, P., Chitmanat, C. & Mangumphan, K. (2014). Appropriate density of recirculating system Nile tilapia culture in aquaponic. Journal of Fisheries Technology Research, 8(1), 23-32.

Kim, D.H., Subramanian, D., & Heo, M.S. (2017). Dietary effect of probiotic bacteria, Bacillus amyloliquefaciens-JFP2 on growth and innate immune response in rock bream Oplegnathus fasciatus, challenged with Streptococcus iniae. Israeli Journal of Aquaculture–Bamidgeh, 1354, 1-11.

Kim, S.S., Galaz, G.B., Pham, M.A., Jang, J.W., Oh, S.H., Yeo, I.K., & Lee, K.J. (2009). Effects of dietary supplementation of a meju, fermented soybean meal, and Aspergillus oryzae for juvenile parrot fish (Oplegnathus fasciatus). Asian-Australasian Journal of Animal Sciences, 22(6), 849-856.

Kühlwein, H., Merrifield, D.L., Rawling, M.D., Foey, A.D., & Davies, S.J. (2014). Effects of dietary β-(1,3)(1,6)-

D-glucan supplementation on growth performance, intestinal morphology and haemato-immunological profile of mirror carp (Cyprinus carpio L.). Journal of Animal Physiology and Animal Nutrition, 98(2),

–289.

Lara-Flores, M., Olvera-Novoa, M.A., Guzmán-Méndez, B.E., & López-Madrid, W. (2003). Use of the bacteria Streptococcus faecium and Lactobacillus acidophilus, and the yeast Saccharomyces cerevisiae as growth promoters in Nile tilapia (Oreochromis niloticus). Aquaculture, 216, 193-201.

Li, P. & Gatlin III, D.M. (2003). Evaluation of brewer’s yeast (Saccharomyces cerevisiae) as a feed supplement for hybrid striped bass (Moronechrysops x M. saxatilis). Aquaculture, 219, 681-692.

Li, P., & Gatlin III, D.M. (2006). Nucleotide nutrition in fish: current knowledge and future applications. Aquaculture, 251, 141–152.

Li, P., Lewis, D.H., & Gatlin III, D.M. (2004). Dietary oligonucleotides from yeast RNA influence immune response and resistance of hybrid striped bass (Morone chrysops × Morone saxatilis) to Strepococcus iniae infection. Fish & Shellfish Immunology, 16(5), 561-569.

Longmutcha, N. Phichai, K. Hanmoungjai, W. &Rojtinnakorn, J. (2015). Use of yeast supplemental diet for Nile tilapia (Oreochromis niloticus). Journal of Fisheries Technology Research, 9(2), 1-11.

Martinéz-Porchas, M., Martínez-Córdova L.R., & Ramos-Enriquez, R. (2009). Cortisol and glucose: reliable indicators of fish stress?. Pan-American Journal of Aquatic Sciences, 4(2), 158-178.

Mazurkiewicz, J., Przybyl, A., & Mroczyk, W. (2005). Supplementing the feed of common carp (Cyprinus carpio L.) juveniles with the biosaf probiotic. Archives Polish Fisheries, 13(2), 171-180.

Mohanty, S.N., Swain, S.K., & Tripathi, S.D. (1996). Rearing of catla (Catla catla Ham) spawn on formulated diets. Journal of Aquaculture in the Tropics, 11, 253-258.

Morgan, J.D., & Iwama, G.K. (1997). Measurement of stress states in the field. In G.K. Iwama, A.D. Pickering, J.P. Summer, & Schreck, C.B. (Eds.), Fish stress and health in aquaculture. (pp. 247-270). Cambridge: Cambridge University Press.

Nandi, A., Banerjee, G., Dan, S.K., Ghosh, K., & Ray, A.K. (2017). Evaluation of in vivo probiotic efficiency of Bacillus amyloliquefaciens in Labeo rohita challenged by pathogenic strain of Aeromonas hydrophila MTCC 1739. Probiotics and Antimicrobial Proteins. Retrieved February 17, 2018, from https://www.ncbi.nlm.nih.gov/pubmed/28744833

Newaj-Fyzul, A., Al-Harbi A.H., & Austin, B. (2014). Review: developments in the use of probiotic for disease control in aquaculture. Aquaculture, 431, 1-11.

Ortuño, J., Cuesta., A., Rodríguez, A., Esteban, M.A., & Meseguer, J. (2002). Oral administration of yeast, Saccharomyces cerevisiae, enhances the cellular innate immune response of gilthead seabream

(Sparus aurata L.). Veterinary Immunology and Immunopathology, 85, 41-50.

Pakhira, C., Nagesh, T.S., Abraham, T.J., Dash, G., & Behera, S. (2015). Stress responses in rohu, Labeo rohita transported at different densities. Aquaculture Reports, 2, 39-45.

Panase, P. & Mengumphan, K. (2015). Growth performance, length-weight relationship and condition factor of backcross and reciprocal hybrid catfish reared in net cages. International Journal of Zoological Research, 11, 57-64.

Park, I.S., Hur, J.W., & Choi, J.W. (2012). Hematological responses, survival, and respiratory exchange in the olive flounder, Paralichthys olivaceus, during starvation. Asian-Australasian Journal of Animal Sciences, 25, 1276–1284.

Perdikaris, C., Nathanailides, C., Gouva, E., Gabriel, U.U., Bitchava, K., Athanasopoulou, F., Paschou, A., & Paschos, I. (2010). Size-relative effectiveness of clove oil as an anaesthetic for rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and goldfish (Carassius auratus Linnaeus, 1758).

ActaVeterinaria Brno, 79, 481-490.

Peterson, B.C., Booth, N.J. & Manning, B.B. (2012). Replacement of fish meal in juvenile channel catfish, Ictalurus punctatus, diets using a yeast-derived protein source: the effects on weight gain, food conversion ratio, body composition and survival of catfish challenged with Edwardsiella ictaluri. Aquaculture Nutrition, 18, 132-137.

Petjul, K. & Khrueasri, T. (2016). Dietary probiotic and effective microorganisms (EM) supplementation on growth performance and survival rate of climbing perch, Anabas testudineus in cage culture. KhonKaen Agriculture Journal, 44 (1), 29-34.

Pongpet, J. (2014). The replacement of fishmeal by brewer’s yeast (Saccharomyces cerevisiae) in diets of Thai Panga. Animal Production Technology, Suranaree University of Technology, 93 p. Retrieved May 10, 2018, from http://sutir.sut.ac.th:8080/sutir/bitstream/123456789/5205/2/Fulltext.pdf

Prado-Rebolle, O.F., García-Márquez, L.J., Macedo-Barragánet, R.J., García-Curiel, J.L., Sánchez-Barajas, M. & Téllez-Isaías, G. (2014). Effects of probiotic and stocking density on Nile tilapia fingerlings (Oreochromis niloticus) productive performance. Revista Científica FCV- LUZ, 24, 180-184.

Ran, C., Huang, L., Hu, J., Tacon, P., He. S., Li, Z., Wang, Y., Liu, Z., Xu, L., Yang, Y., & Zhou, Z. (2016). Effect of dietary live and heat-inactive baker’s yeast on growth, gut health, and disease resistance of Nile tilapia under high rearing density. Fish & Shellfish Immunology, 56, 263-271.

Ran, C., Huang, L., Liu, Z., Xu, L., Yang, Y., Tacon, P., Auclair, E., & Zhou, Z. (2015). A comparison of the beneficial effects of live and heat-inactivated baker’s yeast on Nile tilapia: suggestions on the role and function of the secretory metabolites released from the yeast. PLoS ONE, 10(12), e0145448.

Refstie, S., Baeverfjord, G., Seim, R.R., & ElvebØ, O. (2010). Effects of dietary yeast cell wall β-glucans and MOS on performance, gut health, and salmon lice resistance in Atlantic salmon (Salmo salar) fed sunflower and soybean meal. Aquaculture, 305, 109–116.

Reyes-Cerpa, S., Vallejos-Vidal, E., Gonzalez-Bown, M.J., Morales-Reyes, J., Pérez-Stuardo, D., Vargas, D., Imarai, M., Cifuentes, V., Spencer, E., Sandino, A.M., & Reyes-López, F.E. (2018). Effect of yeast (Xanthophyllomyces dendrorhous) and plant (Saint John's wort, lemon balm, and rosemary) extract based functional diets on antioxidant and immune status of Atlantic salmon (Salmo salar) subjected to crowding stress. Fish & Shellfish Immunology, 74, 250-259.

Ridha, M.T. (2006). Comparative study of growth performance of three strains of Nile tilapia, Oreochromis niloticus, L. at two stocking densities. Aquaculture Research, 37(2), 172-179.

Rodmongkoldee, M., Leelapatra, W., & Thaimuangphol, W. (2017). Effect of probiotics on growth performance and survival rate in Anabas testudineus. Journal of Science & Technology, special issue, 82-89.

Sayed, S.H., Zakaria, A., Mohamed, G.A., & Mohamed, K.K. (2011). Use of probiotics as growth promoter, anti-bacterial and their effects on the physiological parameters and immune response of Oreochromis niloticus Lin. fingerlings. Journal of the Arabian aquaculture society, 6, 201-222.

Selim, K.M. & Reda, R.M. (2015). Beta-glucans and mannan oligosaccharides enhance growth and immunity in Nile tilapia. North American Journal of Aquaculture, 77, 22–30.

Sener, G., Toklu, H., Ercan, F., &Erkanli, G. (2005). Protective effect of beta-glucan against oxidative organ injury in a rat model of sepsis. International immunopharmacology, 5 (9),1387-1396.

Sutthi, N., Thaimuangphol, W., Rodmongkoldee, M., Leelapatra, W., & Panase, P. (2018). Growth performances, survival rate, and biochemical parameters of Nile tilapia (Oreochromis niloticus) reared in water treated with probiotic. Comparative Clinical Pathology, 24, 597-603.

Swain, S.K., Rangacharyulu, P.V., Sarkar, S., & Das, K.M. (1996). Effect of a probiotic supplement on growth, nutrient utilization and carcass composition in mrigal fry. Aquaculture, 4, 29-35.

Taoka, Y., Maeda, H., Jo, J.Y., Jeon, M.J., Bai, S.C., Lee, W.J., Yuge, K., & Koshio, S. (2006). Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system. Fisheries Science, 72(2), 310-321.

Telli, G.S., Ranzani-Paiva, M.J.T., Dias D.C., Sussel, F.R., Ishikawa, C.M., & Tachibana, L. (2014). Dietary administration of Bacillus subtilis on hematology and non-specific immunity of Nile tilapia Oreochromis niloticus raised at different stocking densities. Fish & Shellfish Immunology, 39, 305-311.

Trenzado, C.E., Morales, A.E., & de la Higuera, M. (2006). Physiological effects of crowding in rainbow trout, Oncorhynchus mykiss, selected for low and high stress responsiveness. Aquaculture, 258, 583–593.

Uawonggul, N., Rattanamalee, C. & Daduang, S. (2016). Effects of dietary probiotic supplementation on growth enhancement and immune against Aeromonas in Pangasius bocourti. KKU Science Journal. 44(3)

-517.

Vallejos-Vidal, E., Reyes-Lopez, F., Teles, M., & MacKenzie, S. (2016). The response of fish to immunostimulant diets, Fish. Fish & shellfish immunology, 56, 34–69.

Wang, Y.B. & Gu, Q. (2010). Effect of probiotic on white shrimp (Penaeus vannamei) growth performance and immune response. Marine Biology Research, 6(3), 327-332.

Wendelaar-Bonga, S.E. (1997). The stress response in fish. Physiological Reviews, 77(3), 591-625.

Weyts, F.A., Flik, G., Rombout, J.H., & Verburg-van, K.B.M. (1998). Cortisol induces apoptosis in activated B cell, not in other lymphoid cell of the common carp, Cyprinus carpio L. Developmental and comparative immunology, 22, 551-562.

Wirasorn, K., Klarod, K., Hongsprabhas, P., & Boonsiri, P. (2014). Oxidative Stress, Antioxidant and Cancer. Srinagarind Medical Journal, 29(2), 207-219. (in Thai)

Zhai, Q., Yu, L., Li, T., Zhu, J., Zhang, C., Zhao, J., Zhang, H., & Chen, W. (2017). Effect of dietary probiotic supplementation on intestinal microbiota and physiological conditions of Nile tilapia (Oreochromis niloticus) under waterborne cadmium exposure. Antonie Van Leeuwenhoek, 110, 501-513.


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