Development of a Paper Test Strip for Detection of Lead

Siriwan Teepoo, Sunisa Meenon



This research was the development of paper test for lead detection based on the reaction between lead and alizarin red S resulting in reddish brown product. Then reddish brown intensity was measured by image processing program. The paper test was fabricated by screening the polylacetic acid solution on chromatography paper using screen-printing technique to create hydrophobic barriers on paper. The optimum conditions of paper test for detection of lead were studied such as mole ratio of alizarin red S, pH value and reaction time between lead and alizarin red S. The results were found that the optimum concentration of alizarin red S was 14 mM. The pH value of 4.00 and the reaction time of 1 min were obtained for optimization of the reaction between lead and alizarin red S. Under optimal conditions, the color intensity was linearly correlated with concentration of lead in the range of 0.08 to 2.00 ppm. The detection limit was 0.06 ppm and %recovery was 87-116. The advantage, of the developed paper test strip were high accuracy and precision, easy to use, short analysis time.


Keywords :  lead, paper test strip, chromatography paper, alizarin red S

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Abadin H., Ashizawa, A., & Stevens, Y.W. ( 2007). Toxicological Profile for Lead, Lead. 1-47.

Coco, F. L., Monotti, P., Cozzi, F., & Adami, G. (2006). Determination of cadmium and lead in fruit juices by stripping chronopotentiometry and comparison of two sample pretreatment procedures. Food Control, 17, 966-970.

FDA. (2003). Notification of the Ministry of Public Health (No. 273), 2003, Re: Food Standards Containing Contaminants (No. 2). Government Gazette, 273, 10. (in Thai)

Khalaf F. A. (2011). Spectrophotometric Assay of Lead in Human Hair Samples by using alizarin red(S) in Samarra area. Journal of university of Anbar for pure science, 5(3),

Li, J.X., Sun, C.J., Zheng, L., Jiang, F.H., Yin, X.F., & Chen J.H. (2016). Determination of Lead Species in Algae by Capillary Electrophoresis-Inductively Coupled Plasma-Mass Spectrometry. Chinese Journal of Analytical Chemistry, 44, 1659-1664.

Norton, G. J., Deacon, C. M., Mestrot, A., Feldmann, J., Jenkins, P., & Baskaran, C. (2015). Cadmium and lead in vegetable and fruit produce selected from specific regional areas of the UK. Science of The Total Environment ,533, 520-527.

Satendra P. S. (1965). Composition and Stability of Lead-Alizarin Red S Chelate. Advanced Synthesis & Catalysis, 30, 60-62.

Taverniers, I., Loose, M.D., & Bockstaele, E. V. (2004). Trends in quality in the analytical laboratory. II. Analytical method validation and quality assurance. TrAC Trends in Analytical Chemistry, 23, 535-552.

Zhao, S. L., Chen, F. S., Zhang, J., Ren, S. B., Liang, H. D., & Li, S. S. (2015). On-line flame AAS determination of traces Cd(II) and Pb(II) in water samples using thiol-functionalized SBA-15 as solid phase extractant. Journal of Industrial and Engineering Chemistry, 27, 362-367.

Zhou, Q., Lei, M., Liu, Y., Wu Y., & Yuan, Y. (2017). Simultaneous determination of cadmium, lead and mercury ions at trace level by magnetic solid phase extraction with Fe@Ag@Dimercaptobenzene coupled to high performance liquid chromatography. Talanta, 175, 194-199.



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